Crystal structure of botulinum neurotoxin type G light chain
نویسندگان
چکیده
منابع مشابه
2.3 A crystal structure of tetanus neurotoxin light chain.
TeNT is the causative agent of the neuroparalytic disease tetanus. A key component of TeNT is its light chain, a Zn(2+) endopeptidase that targets SNAREs. Recent structural studies of closely related BoNT endopeptidases indicate that substrate-binding exosites remote from a conserved active site are the primary determinants of substrate specificity. Here we report the 2.3 A X-ray crystal struct...
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Highly purified recombinant zinc-endopeptidase light chain of the botulinum neurotoxin serotype A underwent autocatalytic proteolytic processing and fragmentation. In the absence of added zinc, initially 10-28 residues were cleaved from the C-terminal end of the 448-residue protein followed by the appearance of an SDS-stable dimer and finally fragmentation near the middle of the molecule. In th...
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The botulinum neurotoxins (BoNTs), the most toxic biological compounds, are known to cause muscle paralysis. These toxins are enzymatic properties which inhibit the release of the acetylcholine. The purposes of this study are the recombinant expression of light chain (LC) of botulinum toxin types A (rBoNT/A-LC) with high purity, the evaluation of its antibody titer yield and its immunogenicity,...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section A Foundations of Crystallography
سال: 2005
ISSN: 0108-7673
DOI: 10.1107/s0108767305089142